Acid and Alkaline Phosphatase Activity in the Indian Apple Snail Pila globosa (Swainson) During Aestivation

The Indian apple snail Pila globosa (Swainson) experiences an annual cycle of aestivation (summer sleep) as a survival strategy during a hot and dry period to avoid damage from high temperatures and the risk of desiccation. Alterations in the external environment reflect in their haemolymph. Changes in enzyme levels because of any type of stress are immediately reflected in the functional responses of animals. These environmental factors can be simulated in the laboratory so as to study enzymatic alterations occurring in the haemolymph of snails to overcome the adverse features during aestivation. Phosphatase enzymes are important for many biological functions. Acid and alkaline phosphatase activities have been studied in the haemolymph of three months aestivated and active snails. The activity level of enzymes ACP decreased and ALP increased significantly in the haemolymph of aestivated P. globosa when compared to active snails. The significance of these findings is discussed. This investigation explains the adaptability of P. globosa to overcome the adverse features during aestivation. This study also reveals that Pila globosa is a suitable model for studies on aestivation and provides an interesting case of aestivation

A computational model of ureteral peristalsis and an investigation into ureteral reflux.

The aim of this study is to create a computational model of the human ureteral system that accurately replicates the peristaltic movement of the ureter for a variety of physiological and pathological functions. The objectives of this research are met using our in-house fluid-structural dynamics code (CgLes-Y code). A realistic peristaltic motion of the ureter is modelled using a novel piecewise linear force model. The urodynamic responses are investigated under two conditions of a healthy and a depressed contraction force. A ureteral pressure during the contraction shows a very good agreement with corresponding clinical data. The results also show a dependency of the wall shear stresses on the contraction velocity and it confirms the presence of a high shear stress at the proximal part of the ureter. Additionally, it is shown that an inefficient lumen contraction can increase the possibility of a continuous reflux during the propagation of peristalsis

Earthquake Input Motions and Seismic Site Response in a Centrifuge Test Examining SFSI Effects

This paper describes the ground motion selection process and reports observed seismic site response and SFSI effects during a dynamic centrifuge test (Test-1). The centrifuge test is the first in a series of tests examining the effects of SFSI in dense urban environments. The objective of Test-1 is to examine SFSI effects for two structures that are located a significant distance apart and essentially isolated. The model structures represent a three-story building founded on spread footings and a nine-story structure founded on a threestory basement. The structures are sited on a dry, dense bed of Nevada Sand. The centrifuge model is subjected to a series of shaking events that represent near-fault and “ordinary” ground motions at a site in Los Angeles. Results show that site periods degrade as ground motion intensity increases with more pronounced degradation observed for near-fault ground motions as compared with ordinary ground motions. Additionally, the results indicate the importance of kinematic effects of embedded structures when considering SFSI effects

Seismic Performance Assessment in Dense Urban Environments: Evaluation of Nonlinear Building-Foundation Systems Using Centrifuge Tests

In dense urban areas, buildings are generally constructed in clusters, forming city blocks. New buildings are designed assuming their response is independent of adjacent buildings, which ignores potentially important structure-soil-structure-interaction (SSSI) effects. Although a few studies have revealed the significance of SSSI effects, validated simulation and design tools do not exist. In this paper, we present the results from the first in a series of centrifuge tests intended to investigate SSSI effects. Results herein are focused on the design and measured response of two model building-foundation systems placed on dense dry Nevada sand and tested at 55-g. The two models represent prototypical nine-story and three-story special moment resisting frame buildings, with the former structure supported by a three-level basement-mat and the later on isolated spread footings. Nonlinear response-history simulations are performed to aid in the design of the models, with particular attention to reproducing prototype building periods and nonlinear characteristics. Yielding of the model buildings is achieved using custom-designed fuses placed strategically throughout the superstructures. At present, the two models are placed as far apart as possible to characterize soil-structure interaction on individual buildings; subsequent experiments will move the structures in near proximity, allowing direct experimental assessment of structuresoil- structure-interaction

Faecal calprotectin: factors affecting levels and its potential role as a surrogate marker for risk of development of Crohn's Disease.

BACKGROUND: Faecal calprotectin (FC) is one of the most widely used non-invasive tests for the diagnosis and assessment of Crohn's disease (CD) activity. Despite this, factors other than disease activity which affect levels have not been extensively reviewed. This is of importance when using FC in the diagnostic setting but also may be of utility in studying the aetiology of disease. OBJECTIVES: Our review outlines environmental risk factors that affect FC levels influencing diagnostic accuracy and how these may be associated with risk of developing CD. FC as a surrogate marker could be used to validate risk factors established in case control studies where prospective studies are not feasible. Proof of this concept is provided by our identification of obesity as being associated with elevated FC, our subsequent confirmation of obesity as risk factor for CD and the subsequent verification in prospective studies, as well as associations of lack of physical activity and dietary fibre intake with elevated FC levels and their subsequent confirmation as risk factors in prospective studies. CONCLUSION: We believe that FC is likely to prove a useful surrogate marker for risk of developing CD. This review has given a theoretical basis for considering the epidemiological determinants of CD which to date has been missing

Membrane protein extraction and purification using styrene-maleic acid (SMA) co-polymer:effect of variations in polymer structure

The use of styrene maleic acid (SMA) co-polymers to extract and purify transmembrane proteins, whilst retaining their native bilayer environment, overcomes many of the disadvantages associated with conventional detergent based procedures. This approach has huge potential for the future of membrane protein structural and functional studies. In this investigation we have systematically tested a range of commercially available SMA polymers, varying in both the ratio of styrene to maleic acid and in total size, for the ability to extract, purify and stabilise transmembrane proteins. Three different membrane proteins (BmrA, LeuT and ZipA) which vary in size and shape were used. Our results show that several polymers can be used to extract membrane proteins comparably to conventional detergents. A styrene:maleic acid ratio of either 2:1 or 3:1, combined with a relatively small average molecular weight (7.5-10 kDa) is optimal for membrane extraction, and this appears to be independent of the protein size, shape or expression system. A subset of polymers were taken forward for purification, functional and stability tests. Following a one-step affinity purification SMA 2000 was found to be the best choice for yield, purity and function. However the other polymers offer subtle differences in size and sensitivity to divalent cations that may be useful for a variety of downstream applications